M5948
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 | Immunoblotting Lysates of various cell lines were separated on SDS-PAGE and blotted with 1 μg/mL Rabbit Anti-MIB1 (C-terminal) (Cat. No. M5948). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545), and a chemiluminiscence substrate. Lanes: 1. A431; 2. A549; 3. 293-T over expressing human MIB1; 4. PC-12 |
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M5948
Sigma
Anti-MIB1 (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-DAPK-interacting protein 1, Anti-DIP-1, Anti-KIAA1323, Anti-Mindbomb homolog 1, Anti-Ubiquitin ligase mind bomb |
Description
| Immunogen | synthetic peptide corresponding to amino acids 924-939 of human MIB1, conjugated to KLH. The corresponding sequence is identical in rat and mouse. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~110 kDa |
| species reactivity | rat, human, mouse |
| concentration | ~1.0 mg/mL |
| application(s) | immunoblotting: 0.5-1 μg/mL using lysates of A549 or PC12 cells. |
| Gene Information | human ... MIB1(57534) |
| rat ... Mib1(307594) | |
| mouse ... Mib1(225164) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 1 |
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| M6073, Anti-MIB1 (N-terminal) antibody produced in rabbit |
